The largest increases following stimulation with either H5N1 virus or CD2/CD2R relative to the media control was for IFN-γ. nSFC levels for IFN-γ were strongly correlated (ρ > 0.72) with IFN-γ concentrations measured from the corresponding supernatant for all timepoints and for both H5N1 Tasquinimod (Table 3, Supplemental Fig. 2). Within the primed group, the younger age group had higher correlations compared to the older age group following Clade 1 virus stimulation (Supplementary Table 3). IFN-γ concentrations also correlated with GrB nSFCs levels, but less strongly (rs > 0.42). Positive correlations between nSFC levels and concentrations of other cytokines were observed, but correlation coefficients were lower or were not statistically significant at all timepoints (Table 3). IL-6 concentrations in PBMC supernatants were negatively correlated with nSFC responses at some timepoints.
Table 3.
Significant correlations between nSFC per 2 × 105 PBMCs and normalized cytokine levels measured in PBMC supernatants following stimulation with H5N1 virusesa.aColor scheme: Dark orange, ρ ≥ 0.75; medium orange, 0.75 > ρ > 0. 5; light orange, 0.50 ≥ ρ>0; light blue, −0.50 ≤ ρ <0. Only significant (P < 0.05) correlations are bicarbonate ions shown.Full-size tableTable optionsView in workspaceDownload as CSV
Table 3.
Significant correlations between nSFC per 2 × 105 PBMCs and normalized cytokine levels measured in PBMC supernatants following stimulation with H5N1 virusesa.aColor scheme: Dark orange, ρ ≥ 0.75; medium orange, 0.75 > ρ > 0. 5; light orange, 0.50 ≥ ρ>0; light blue, −0.50 ≤ ρ <0. Only significant (P < 0.05) correlations are bicarbonate ions shown.Full-size tableTable optionsView in workspaceDownload as CSV